Journal: Leukemia

Article Title: HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3.

doi: 10.1038/sj.leu.2403716

Figure Lengend Snippet: Figure 3 Expression of IL6 and HLXB9 by RT-PCR in response to HLXB9 modulation. (a) Western blot control of expression vector pcrHLXB9 in comparison to empty vector pcDNA3 transfected in 293 cells. GDM-1 positive control cells strongly express HLXB9.29 (b) HL cell line L-540 was treated with 107 M TPA or 0.1% DMSO in combination with transfection of pcrHLXB9. Expression of IL6 is detectable after TPA treatment only. Transfection with pcrHLXB9 enhances expression of IL6. (c) HDLM-2 cells were treated with antisense-oligo (AS) directed against HLXB9 in comparison to control- oligo (AS-control). Both HLXB9 and IL6 evidenced reduced expres- sion. NTC: no template control.

Article Snippet: Inhibition of secreted IL6 protein was performed using anti-IL6 antibody (R&D Systems, Wiesbaden, Germany) and for stimulation experiments IL6 protein was purchased from Strathmann Biotec AG (Hamburg, Germany).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Plasmid Preparation, Comparison, Transfection, Positive Control

Journal: Leukemia

Article Title: HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3.

doi: 10.1038/sj.leu.2403716

Figure Lengend Snippet: Figure 4 Schematic diagram of IL6 promoter constructs. IL6 promoter sequences were cloned before a reporter gene (EGFP). Potential binding sites for AP-1, NFkB, and GBX2/HLXB9 (EHG) are indicated.

Article Snippet: Inhibition of secreted IL6 protein was performed using anti-IL6 antibody (R&D Systems, Wiesbaden, Germany) and for stimulation experiments IL6 protein was purchased from Strathmann Biotec AG (Hamburg, Germany).

Techniques: Construct, Clone Assay, Binding Assay